CPT (Certified Phlebotomy Technician) Practice Exam 2025 – 400 Free Practice Questions to Pass the Exam

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What does “inverting” a collection tube mean?

Rolling the tube back and forth

Gently turning it over to mix the contents without shaking

Inverting a collection tube refers to the process of gently turning the tube over to mix the contents without shaking. This action is essential after blood is collected to ensure that any additives present in the tube, such as anticoagulants or preservatives, are evenly distributed throughout the blood sample. Inverting helps to avoid clotting or other reactions that might occur if the sample is not mixed properly.

Rolling the tube back and forth, while it can mix contents, is not typically referred to as inverting and may not sufficiently mix additives. Keeping the tube upright while filling does not relate to the mixing process once the sample is collected. Shaking the tube vigorously can damage blood cells and produce bubbles, compromising the integrity of the sample. Therefore, gently turning the tube over is the most appropriate method for ensuring proper mixing of the collected sample.

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Keeping it upright while filling

Shaking it vigorously to mix

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